How DNA Emerged as a Promising Scientific Tool
Between 1944 and 2024, the search for over 40,000 missing American military personnel at sea spurred research that uses environmental DNA (eDNA) to locate human remains in shipwrecks, thereby expanding investigative methods of the Defense POW/MIA Accounting Agency (DPAA) and strengthening underwater strategies.
DNA gathers genetic fragments released in water and sediments; the technique allows for the detection of degraded human DNA without immediate excavations.
Thus, the team from the Woods Hole Oceanographic Institution (WHOI) began collections between 2022 and 2023 in Saipan, in Lake Huron, and in the Mediterranean.
The goal was to differentiate ancient sequences from recent sequences.
Similar research has existed since 2008, when French scientists detected the invasive bullfrog through eDNA.
This was the first test directly applied to military wrecks.
The Case of the Avenger Shot Down in the Battle of Saipan in 1944
A Grumman TBF Avenger, shot down during the Battle of Saipan in 1944, became the focus of the study because two crew members remain missing.
The coral-encrusted plane lies in shallow lagoon waters.
This location allowed for the collection of sediments and water by Calvin Mires, a marine archaeologist from WHOI.
The researchers identified short fragments of human DNA.
The fragments suggest a possible presence of human remains.
How Samples Were Collected and Analyzed
The team faced logistical challenges during collections in 2022 and 2023.
The airline refused to transport the samples with dry ice.
However, the samples were taken to Guam and stored properly.
They were then sent to the Biotechnology Center at the University of Wisconsin.
Charles Konsitzke confirmed that the preservation remained adequate.
Between 2023 and 2024, researchers conducted complete metagenomic sequencing.
They separated fragments shorter than 150 base pairs.
These fragments represent ancient DNA, as explained by Kirstin Meyer-Kaiser from WHOI.
Key Findings and Unexpected Patterns
Locations in Saipan and Palermo showed a high abundance of short fragments.
However, cold and deep environments like Lake Huron exhibited more ancient human DNA at control sites than at suspected areas.
Geneticist Elena Irene Zavala from the University of Copenhagen suggested that human DNA is widely dispersed in the marine environment.
She noted that preservation is greater in low-temperature areas.
Microbial patterns revealed genes linked to glycosaminoglycans.
These molecules are common in animal tissues.
Limitations, Need for Excavation, and Next Steps
No human remains appeared during the study, thus preventing definitive conclusions about the match between fragments and remains.
Therefore, additional excavations become necessary to confirm areas of higher genetic abundance.
The report delivered in April 2024 recommended interventions at three sites, although the DPAA is still assessing continuity.
Meanwhile, Jesse Stephen, the DPAA’s Director of Innovation, stated that eDNA serves as an investigative tool, thus unable to identify individuals, but useful for prioritizing critical regions.

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